Cytosol Peroxiredoxin and Cell Surface Catalase Differentially Respond to H2O2 Stress in Aspergillus nidulans

Both catalase and peroxiredoxin show high activities of H2O2 decomposition coexist in the same organism; however, their division labor defense against is unclear. We focused on major (PrxA) (CatB) Aspergillus nidulans at different growth stages to discriminate antioxidant roles. The dormant conidia lacking PrxA showed sensitivity concentrations (>100 mM), revealing that one important antioxidants conidia. Once began swell germinate, or further develop young hyphae (9 h old age), PrxA-deficient cells (ΔprxA) did not survive plates containing higher than 1 mM, indicating an indispensable early stage. During these stages, absence CatB affect fungal resistance either (>1 mM) low (<1 H2O2. In mature stage (24 fulfills function, especially doses constitutively expressed throughout lifespan, whereas levels are developing from swelling hyphae, providing a molecular basis for contributions stages. Further enzyme activity cellular localization analysis indicated needs be secreted functionalized, this process confined hyphae. Our results revealed differences effectiveness timelines two primary anti-H2O2 enzymes fungus.